OPEN (Oligomerized Pool ENgineering) Zinc Finger Pools, Vectors, and Strains

Requests for the reagents described in the Maeder et al., Molecular Cell 2008 paper should be directed to Keith Joung (JJOUNG@PARTNERS.ORG). Vector sequences and maps will be made available online in the near future. Please check back here for updates in the near future.

Zinc Finger Consortium Modular Assembly Kit v1.0

This kit consists of 141 plasmids each encoding a different zinc finger module from one of three previously described archives. Plasmids encoding these modules have been constructed on a standardized Zinc Finger Consortium platform which permits facile "mixing and matching" of these fingers using only standard restriction digest-based subcloning methods (references: Wright et al., Nature Protocols 2006 and Ramirez et al., Nature Methods 2008 [see Publications]). Multi-finger arrays assembled in this framework can be readily transferred to various Consortium expression vectors that permit assessment of DNA-binding activities in a bacterial cell-based two-hybrid system and expression as zinc finger nucleases (ZFNs) in various cell types (see Zinc Finger Consortium Expression Vector Kit below). This archive of modules is available as a set from Addgene, a non-profit plasmid distribution service (see http://www.addgene.org/zfc for ordering details). Full plasmid maps and sequences are also available on the Addgene website <http://www.addgene.org/zfc>. Important information regarding anticipated success rates when using these modules to create ZFNs can be found in a recent publication from the ZFC (Ramirez et al., Nature Methods 2008 -- see Publications).

Zinc Finger Consortium Expression Vector Kit v1.0

This kit consists of plasmids and a bacterial strain needed to: (1) test the activity of assembled zinc finger arrays in a bacterial cell-based assay and (2) express ZFNs in plant or mammalian cells (reference: Wright et al., Nature Protocols 2006 [see Publications]). These reagents are available either individually or as a set from Addgene, a non-profit plasmid distribution service (see http://www.addgene.org/zfc for ordering details.) Full plasmid maps and sequences are also available on the Addgene website <http://www.addgene.org/zfc>.